Choline acetyltransferase binding to and release from membranes
نویسندگان
چکیده
منابع مشابه
Choline acetyltransferase from rat brain.
The localization, purification, and some enzymatic properties of choline acetyltransferase from rat brain were studied. Most assays were performed with a specific radiometric micromethod. Solubilization of the enzyme was examined after homogenization of cerebral cortices by means which disintegrate nerve endings. In isotonic KC1 the enzyme was recovered in solution. In more dilute KC1 the enzym...
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Immunohistochemical localization of choline acetyltransferase (ChAT) in cholinergic neurons has been difficult to achieve because of problems encountered in producing specific antisera. Here we describe the production and characterization of several distinct monoclonal antibodies to ChAT. Each of the monoclonal antibodies exhibits one of three general patterns of cross-species reactions; one pa...
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Choline acetyltransferase (ChAT), the enzyme that synthesizes the neurotransmitter acetylcholine (ACh), is thought to be present in kinetic excess in cholinergic neurons. The rate-limiting factor in ACh production is the provision of choline to ChAT. Cholinergic neurons are relatively unique in their expression of the choline transporter 1 (CHT1), which exhibits high-affinity for choline and ca...
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The effects of semi-chronic stress on neuronal morphology and choline acetyltransferase were studied by injecting rats with dexamethasone. It was found that in the dexamethasone-treated rats the choline acetyltransferase activity had increased in the area where the fibers from the perforant pathway synapse with the granular cell layer of the dentate gyrus and also where the mossy fibers of the ...
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Mutations in the superoxide dismutase 1 (sod1) gene cause familial amyotrophic lateral sclerosis (FALS), likely due to the toxic properties of misfolded mutant SOD1 protein. Here we demonstrated that, starting from the pre-onset stage of FALS, misfolded SOD1 species associates specifically with kinesin-associated protein 3 (KAP3) in the ventral white matter of SOD1(G93A)-transgenic mouse spinal...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1968
ISSN: 0306-3283
DOI: 10.1042/bj1090389